引用文献：Scientia_Horticulturae, 114(2):121-128 (2007).
Mark S. Roha, Eun Ju Cheongb, Ik-Young Choic and Young Hee Joungd
aUS Department of Agriculture, Agricultural Research Service, National Arboretum, Floral and Nursery Plants Research Unit, Beltsville, MD 20705, USA
bUS Department of Agriculture, Agricultural Research Service, National Germplasm Resources Laboratory, Plant Disease Research Unit, Beltsville, MD 20705, USA
cSeoul National University, CALS, NICEM, San 56-1 Sillim-9-dong Gwanak-gu, Seoul 151-921, Republic of Korea
dCheonnam National University, School of Biological Science & Technology, Gwangju 500-757, Republic of Korea
原文要旨
This study was initiated to attempt clarify the identities of taxa referred to as Prunus yedoensis that grows under natural environments in Jeju, Korea and of Yoshino cherry hybrids of cultivated origin (also recorded as P. × yedoensis) in Japan, and to understand the difference between these two taxa. P. yedoensis and other species collected from natural habitats from Jeju, Korea and cultivated materials of Yoshino cherries from Tokyo and Washington, DC. were analyzed with inter-simple sequence repeat (ISSR) markers, and sequence analysis of two chloroplast DNA (cpDNA) genes, rpl16 and trnL-trnF spacer. Depending on the source of Yoshino cherry, accessions show variations with ISSR and cpDNA. Accessions belonging to each of P. serrulata var. spontanea, P. serrulata var. pubescens, and P. sargentii were grouped closely to P. yedoensis and Yoshino cherry accessions. However, two Yoshino cherry accessions that include ‘Akebono’ showed the same rpl16 haplotype of A and A at the position of 113 and 206, respectively, which were found in 4 out of 16 P. yedoensis accessions. Twelve accessions of P. yedoensis and 11 other Yoshino cherries showed rpl16 haplotype of T and A at these positions. P. yedoensis native to Korea can be considered different from Yoshino cherry of hybrid origin from Japan based on ISSR markers and rpl16 haplotypes. Therefore, it may be concluded that the Korean taxon currently referred to as P. yedoensis can be considered indigenous and sufficiently distinct to warrant recognition as a distinct entity.
要旨日本語訳
本研究は、韓国済州島の自然環境の下で生育する Prunus yedoensisと称される分類群と、日本の栽培起源であるソ¥メイヨシノ分類群（Ｐ.× yedoensisとも表¥記される）との正体を明らかにするために、開始したものである。韓国済州島の自然生態から採集されたP. yedoensisとその他の種、および東京とワシントンＤＣから採集されたソ¥メイヨシノの栽培品を、ISSR (inter-simple sequence repeat)マーカーおよびchloroplast ＤＮＡ（cpDNA）遺伝子であるrpl16と trnL-trnFスペーサーの連続分析で解析した。 ソ¥メイヨシノのソ¥ースによってはISSRとcpDNA遺伝子のアクセッションに変異が見られた。P. serrulata var. spontanea（ヤマザクラ）、Ｐ. serrulata var. pubescens（カスミザクラ）とＰ sargentii（オオヤマザクラ）のそれぞれに属するアクセッションはＰ. yedoensisおよびソ¥メイヨシノのアクセッションと緊密なグループであることがわかった。 しかしながら、アケボノ（曙）を含む２つのソ¥メイヨシノのアクセッションは、それぞれ113と206のポジションにおいて、同じＡのrpl16ハロタイプおよびＡを示し、16あるP. yedoensisアクセッションのうちの4つに見られた。Ｐ. yedoensisの残りの12のアクセッションおよびその他の11種のソ¥メイヨシノはこのポジションでＴとＡのrpl16ハロタイプを示した。韓国原産のP. yedoensisは、 ISSR マーカーおよびrpl16 ハロタイプに基づけば、日本の交配種起源のソ¥メイヨシノとは異なると考えられる。 したがって、一般にＰ. yedoensisと称される韓国の分類群は（韓国に）固有であり、別種として認識するに足ると考えられる。
引用文献：Jpn. J. Genet. (1995) 70, pp. 185-196.
Hideki INNAN1, Ryohei TERAUCHI2, Naohiko T. MIYASHITA1 and Koichiro TSUNEWAKI1
1Laboratory of Genetics, Faculty of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-01
2Department of Botany, Faculty of Science, Kyoto University, Sakyo-ku, Kyoto 606-01, Japan
原文要旨
In order to investigate the intraspecific variation of Prunus yedoensis (Someiyoshino) and interspecific relationship among P. yedoensis, P. lannesiana (Oshimazakura) and P. pendula (Edohigan), DNA fingerprinting study was conducted by using two different kinds of probes, M13 repeat sequence and (GACA)4 synthetic oligonucleotide. In this study, 68 plants of P. yedoensis grown in 46 prefectures in Japan were investigated. All the P. yedoensis individuals investigated showed the completely same banding pattern, indicating their clonal origin from a single plant. On the other hand, each of P. lannesiana and P. pendula individuals investigated showed a unique banding pattern, suggesting a considerable amount of genetic variation in these two species. About 90% of bands in DNA fingerprints of P. yedoensis were detected in either P. lannesiana or P. pendula. This result supports the hypothesis that P. yedoensis is an interspecific hybrid between P. lannesiana and P. pendula. From those results, it is concluded that P. yedoensis was produced only once through hybridization between P. lannesiana and P. pendula, and that this particular hybrid plant has been spread vegetatively all over Japan.
要旨日本語訳
Prunus yedoensis（ソ¥メイヨシノ）の種内変異およびP. yedoensis、Ｐ. lannesiana（オオシマザクラ）とＰ pendula（エドヒガン）の間の種間の関係を調べるために、異なった２種類のプローブ、すなわちM13 repeat sequenceと(GACA)4 synthetic oligonucleotide を用いて、ＤＮＡによる指紋鑑定研究を行った。 この研究では、日本の４６都道府県で植栽するＰ. yedoensisの68個体を調べた。調査したＰ. yedoensisの個体のすべては、完全に同じバンドパターンを示し、（ソ¥メイヨシノが）一つの個体からのクローンに起源することを示している。一方、 調べたＰ. lannesiana とＰ. Pendulaの個体はそれぞれユニークなバンドパターンを示し、この２種ではかなりの遺伝変異のあることを示唆する。Ｐ. yedoensis のＤＮＡ指紋のバンドのおよそ９０％はＰ. lannesianaあるいはＰ. pendulaで検出された。この結果はＰ. yedoensis がＰ. lannesiana とＰ. pendula との種間交雑であるという仮説を支持する。 以上の結果から、Ｐ. yedoensis はＰ. lannesiana とＰ. pendula の間の交雑を通してただ１度だけ生成したものであり、この交雑種が日本中に無性的繁殖で広がったと結論づけられる。
인용 문헌：Scientia_Horticulturae, 114(2):121-128 (2007).
Mark S. Roha, Eun Ju Cheongb, Ik-Young Choic and Young Hee Joungd
aUS Department of Agriculture, Agricultural Research Service, National Arboretum, Floral and Nursery Plants Research Unit, Beltsville, MD 20705, USA
bUS Department of Agriculture, Agricultural Research Service, National Germplasm Resources Laboratory, Plant Disease Research Unit, Beltsville, MD 20705, USA
cSeoul National University, CALS, NICEM, San 56-1 Sillim-9-dong Gwanak-gu, Seoul 151-921, Republic of Korea
dCheonnam National University, School of Biological Science & Technology, Gwangju 500-757, Republic of Korea
원문 요지
This study was initiated to attempt clarify the identities of taxa referred to as Prunus yedoensis that grows under natural environments in Jeju, Korea and of Yoshino cherry hybrids of cultivated origin (also recorded as P. × yedoensis) in Japan, and to understand the difference between these two taxa. P. yedoensis and other species collected from natural habitats from Jeju, Korea and cultivated materials of Yoshino cherries from Tokyo and Washington, DC. were analyzed with inter-simple sequence repeat (ISSR) markers, and sequence analysis of two chloroplast DNA (cpDNA) genes, rpl16 and trnL-trnF spacer. Depending on the source of Yoshino cherry, accessions show variations with ISSR and cpDNA. Accessions belonging to each of P. serrulata var. spontanea, P. serrulata var. pubescens, and P. sargentii were grouped closely to P. yedoensis and Yoshino cherry accessions. However, two Yoshino cherry accessions that include ‘Akebono＇ showed the same rpl16 haplotype of A and A at the position of 113 and 206, respectively, which were found in 4 out of 16 P. yedoensis accessions. Twelve accessions of P. yedoensis and 11 other Yoshino cherries showed rpl16 haplotype of T and A at these positions. P. yedoensis native to Korea can be considered different from Yoshino cherry of hybrid origin from Japan based on ISSR markers and rpl16 haplotypes. Therefore, it may be concluded that the Korean taxon currently referred to as P. yedoensis can be considered indigenous and sufficiently distinct to warrant recognition as a distinct entity.
요지 일본어 번역
본연구는, 한국 제주도의 자연 환경아래에서 생육하는 Prunus yedoensis라고 칭해지는 분류군과 일본의 재배 기원인 소메이요시노 분류군(P.× yedoensis와도 겉(표)기록된다)와의 정체를 분명히 하기 위해서, 개시한 것이다.한국 제주도의 참마태로부터 채집된 P. yedoensis와 그 외의 종, 및 도쿄와 워싱턴 DC로부터 채집된 소메이요시노의 재배품을, ISSR (inter-simple sequence repeat) 마커 및 chloroplast DNA(cpDNA) 유전자인 rpl16와 trnL-trnF 스페이서-의 연속 분석으로 해석했다. 소메이요시노의 소스에 따라서는 ISSR와 cpDNA 유전자의 아크셋션에 변이를 볼 수 있었다.P. serrulata var. spontanea(야마자크라), P. serrulata var. pubescens(가스미더 곳간)와 P sargentii(장미과 낙엽저목)의 각각 속하는 아크셋션은 P. yedoensis 및 소메이요시노의 아크셋션과 긴밀한 그룹인 것을 알 수 있었다. 그렇지만, 아케보노(새벽)를 포함한 2개의 소메이요시노의 아크셋션은, 각각 113으로 206의 포지션에 대하고, 같은 A의 rpl16 할로 타입 및 A를 나타내, 16있는 P. yedoensis 아크셋션 중 4개로 보여졌다.P. yedoensis의 나머지의 12의 아크셋션 및 그 외의 11종의 소메이요시노는 이 포지션으로 T와 A의 rpl16 할로 타입을 나타냈다.한국 원산의 P. yedoensis는, ISSR 마커 및 rpl16 할로 타입에 근거하면, 일본의 교배종 기원의 소메이요시노와는 다르다고 생각할 수 있다. 따라서, 일반적으로 P. yedoensis라고 칭해지는 한국의 분류군은(한국에) 고유하고, 별종으로서 인식하는에 충분하다고 생각할 수 있다.
인용 문헌：Jpn. J. Genet. (1995) 70, pp. 185-196.
Hideki INNAN1, Ryohei TERAUCHI2, Naohiko T. MIYASHITA1 and Koichiro TSUNEWAKI1
1Laboratory of Genetics, Faculty of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-01
2Department of Botany, Faculty of Science, Kyoto University, Sakyo-ku, Kyoto 606-01, Japan
원문 요지
In order to investigate the intraspecific variation of Prunus yedoensis (Someiyoshino) and interspecific relationship among P. yedoensis, P. lannesiana (Oshimazakura) and P. pendula (Edohigan), DNA fingerprinting study was conducted by using two different kinds of probes, M13 repeat sequence and (GACA)4 synthetic oligonucleotide. In this study, 68 plants of P. yedoensis grown in 46 prefectures in Japan were investigated. All the P. yedoensis individuals investigated showed the completely same banding pattern, indicating their clonal origin from a single plant. On the other hand, each of P. lannesiana and P. pendula individuals investigated showed a unique banding pattern, suggesting a considerable amount of genetic variation in these two species. About 90% of bands in DNA fingerprints of P. yedoensis were detected in either P. lannesiana or P. pendula. This result supports the hypothesis that P. yedoensis is an interspecific hybrid between P. lannesiana and P. pendula. From those results, it is concluded that P. yedoensis was produced only once through hybridization between P. lannesiana and P. pendula, and that this particular hybrid plant has been spread vegetatively all over Japan.
요지 일본어 번역
Prunus yedoensis(소메이요시노)의 종내 변이 및 P. yedoensis, P. lannesiana(오오시마자크라)와 P pendula(에드히간)의 사이의 종간의 관계를 조사하기 위해서, 다른 2 종류의 프로브, 즉 M13 repeat sequence와(GACA)4 synthetic oligonucleotide 를 이용하고, DNA에 의한 지문 감정 연구를 실시했다. 이 연구에서는, 일본의 46 도도부현에서 재배하는 P. yedoensis의 68 개체를 조사했다.조사한 P. yedoensis의 개체의 모든 것은, 완전하게 같은 밴드 패턴을 나타내, (소메이요시노가) 하나의 개체로부터의 클론에 기원 하는 것을 나타내고 있다.한편, 조사한 P. lannesiana 와 P. Pendula의 개체는 각각 독특한 밴드 패턴을 나타내, 이 2종에서는 상당한 유전 변이가 있는 것을 시사한다.P. yedoensis 의 DNA 지문의 밴드의 대략 90%는 P. lannesiana 혹은 P. pendula로 검출되었다.이 결과는 P. yedoensis 가 P. lannesiana 와 P. pendula 와의 종간 교잡이다고 하는 가설을 지지한다. 이상의 결과로부터, P. yedoensis 는 P. lannesiana 와 P. pendula 의 사이의 교잡을 통해 단지 1도만 생성한 것이어, 이 교잡종이 일본안에 무성적 번식으로 퍼졌다고 결론 지을 수 있다.